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Several properties of agar render it a superior jelly. Agar isn’t broken down by microbial enzymes apart from a few species (including bacteria living in marine and freshwater habitats), and it dissolves well in boiling water, making it easy to sterilize. The jelly doesn’t react with the ingredients of a broth, whose composition can be adjusted to meet the nutritional requirements of different microbes, and sets to a firm gel without the need for refrigeration.

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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?